S. Typhi and Bangkok surveillance

Genetic environment of common beta-lactam resistance gene in Salmonella Typhi and Analyzing toilet waste in Bangkok

Presentation from the Research Group for Genomic Epidemiology – 21 November 2022

Genetic environment of blaTEM-1B in global Salmonella Typhi

Salmonella Typhi (S. Typhi) causes typhoid fever, a significant healthcare concern in humans. Antimicrobial resistance in S. Typhi challenges therapeutic treatment. The global reports showed that blaTEM-1B was the most common beta-lactam resistance gene in Salmonella enterica. The objective of this study was to investigate the genetic environment of blaTEM-1B to gain understanding about gene arrangements and its dissemination. The results indicated that blaTEM-1B gene in S. Typhi could be found in various arrangements. At least two Insertion sequences (ISEcp1 and IS26) are involved in dissemination of this gene in S. Typhi affecting beta-lactam resistance worldwide.

Metagenomic Analysis of Toilet Waste from Three Different Income Areas in Bangkok, Thailand

The European Centre for Disease Prevention and Control (ECDC) has weekly reported communicable diseases causing public health threats. In response to the epidemic (outbreak), endemic, or pandemic of (re-)emerging diseases, a surveillance system is an essential tool and an important source of information for responding to infection situations. Wastewater was used for the surveillance of antimicrobial resistance (AMR) in the human population. A high abundance of antimicrobial resistance genes (ARGs) carried on mobile genetic elements in wastewater, a risk factor for AMR transferring mechanism via horizontal gene transfer (HGT). We are interested in conducting of local surveillance of pathogens and AMR using metagenomic-based surveillance from toilet waste in Bangkok, Thailand. The information is useful for epidemiologists and health professionals for national concerns and conducts further proper surveillance.

Narong Nuanmuang's presentation